Chebulagic acid, a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz., induces apoptosis in COLO-205 cell line
Language: 
English
Abstract: 

ETHNOPHARMACOLOGICAL RELEVANCE: Terminalia chebula has an esteemed origin in Indian mythology; its fruits are used to treat many diseases such as digestive, diabetes, colic pain, chronic cough, sore throat, asthma, etc. AIM OF THE STUDY: The water or ethanolic extracts of the fruits were reported to have anti-oxidant, anti-inflammatory, anti-cancer and radio-protector properties. The present study is to isolate and identify the compounds that inhibit COX and 5-LOX, the key enzymes involved in inflammation and carcinogenesis. MATERIALS AND METHODS: The ethanolic extract of the fruits was fractionated by RP-HPLC and fractions were tested for enzyme inhibition activity against COX and 5-LOX. One of the fractionated compounds showed potent dual inhibition against COX and 5-LOX. It was identified as chebulagic acid by LC-MS, NMR and IR analyses. The chebulagic acid was also tested for anti-proliferative activity. RESULTS: Chebulagic acid showed potent COX-LOX dual inhibition activity with IC(50) values of 15+/-0.288, 0.92+/-0.011 and 2.1+/-0.057 microM for COX-1, COX-2 and 5-LOX respectively. It also showed anti-proliferative activity against HCT-15, COLO-205, MDA-MB-231, DU-145 and K562 cell lines. Further mechanistic studies on COLO-205 cells revealed induction of apoptosis by chebulagic acid. CONCLUSIONS: Chebulagic acid, a COX-2 and 5-LOX dual inhibitor isolated from the fruits of Terminalia chebula, induces apoptosis in COLO-205 cells.

Author(s): 
Reddy, D. Bharat
Reddy, T. C. M.
Jyotsna, G.
Sharan, Satish
Priya, Nalini
Lakshmipathi, V.
Reddanna, Pallu
Item Type: 
Journal Article
Publication Title: 
Journal of Ethnopharmacology
Journal Abbreviation: 
J Ethnopharmacol
Publication Date: 
7/30/2009
Publication Year: 
2009
Pages: 
506-512
Volume: 
124
Issue: 
3
ISSN: 
1872-7573
DOI: 
10.1016/j.jep.2009.05.022
Library Catalog: 
PubMed
Extra: 
PMID: 19481594

Turabian/Chicago Citation

D. Bharat Reddy, T. C. M. Reddy, G. Jyotsna, Satish Sharan, Nalini Priya, V. Lakshmipathi and Pallu Reddanna. 7/30/2009. "Chebulagic acid, a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz., induces apoptosis in COLO-205 cell line." Journal of Ethnopharmacology 124: 3: 506-512. 10.1016/j.jep.2009.05.022.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.jep.2009.05.022 | issn = 1872-7573 | volume = 124 | pages = 506-512 | last = Reddy | first = D. Bharat | coauthors = Reddy, T. C. M., Jyotsna, G., Sharan, Satish, Priya, Nalini, Lakshmipathi, V., Reddanna, Pallu | title = Chebulagic acid, a COX-LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz., induces apoptosis in COLO-205 cell line | journal = Journal of Ethnopharmacology | date = 7/30/2009 | pmid = | pmc = }} </ref>