Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells: combinational approach for enhanced differentiation
Language: 
English
Short Title: 
Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells
Abstract: 

Ashwagandha (Withania somnifera) is widely used in the Indian traditional system of medicine, Ayurveda. Although it is claimed to have a large variety of health-promoting effects, including therapeutic effects on stress and disease, the mechanisms of action have not yet been determined. In the present study, we aimed to investigate the growth inhibition and differentiation potential of the alcoholic extract of Ashwagandha leaves (i-Extract), its different constituents (Withaferin A, Withanone, Withanolide A) and their combinations on glioma (C6 and YKG1) cell lines. Withaferin A, Withanone, Withanolide A and i-Extract markedly inhibited the proliferation of glioma cells in a dose-dependent manner and changed their morphology toward the astrocytic type. Molecular analysis revealed that the i-Extract and some of its components caused enhanced expression of glial fibrillary acidic protein, change in the immunostaining pattern of mortalin from perinuclear to pancytoplasmic, delay in cell migration, and increased expression of neuronal cell adhesion molecules. The data suggest that the i-Extract and its components have the potential to induce senescence-like growth arrest and differentiation in glioma cells. These assays led us to formulate a unique combination formula of i-Extract components that caused enhanced differentiation of glial cells.

Author(s): 
Shah, Navjot
Kataria, Hardeep
Kaul, Sunil C.
Ishii, Tetsuro
Kaur, Gurcharan
Wadhwa, Renu
Item Type: 
Journal Article
Publication Title: 
Cancer Science
Journal Abbreviation: 
Cancer Sci.
Publication Date: 
Sep 2009
Publication Year: 
2009
Pages: 
1740-1747
Volume: 
100
Issue: 
9
ISSN: 
1349-7006
DOI: 
10.1111/j.1349-7006.2009.01236.x
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 19575749

Turabian/Chicago Citation

Navjot Shah, Hardeep Kataria, Sunil C. Kaul, Tetsuro Ishii, Gurcharan Kaur and Renu Wadhwa. Sep 2009. "Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells: combinational approach for enhanced differentiation." Cancer Science 100: 9: 1740-1747. 10.1111/j.1349-7006.2009.01236.x.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1111/j.1349-7006.2009.01236.x | issn = 1349-7006 | volume = 100 | pages = 1740-1747 | last = Shah | first = Navjot | coauthors = Kataria, Hardeep, Kaul, Sunil C., Ishii, Tetsuro, Kaur, Gurcharan, Wadhwa, Renu | title = Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells: combinational approach for enhanced differentiation | journal = Cancer Science | date = Sep 2009 | pmid = | pmc = }} </ref>