Fibroblast cytoskeletal remodeling induced by tissue stretch involves ATP signaling

Fibroblasts in whole areolar connective tissue respond to static stretching of the tissue by expanding and remodeling their cytoskeleton within minutes both ex vivo and in vivo. This study tested the hypothesis that the mechanism of fibroblast expansion in response to tissue stretch involves extracellular ATP signaling. In response to tissue stretch ex vivo, ATP levels in the bath solution increased significantly, and this increase was sustained for 20 min, returning to baseline at 60 min. No increase in ATP was observed in tissue incubated without stretch or tissue stretched in the presence of the Rho kinase inhibitor Y27632. The increase in fibroblast cross sectional area in response to tissue stretch was blocked by both suramin (a purinergic receptor blocker) and apyrase (an enzyme that selectively degrades extracellular ATP). Furthermore, connexin channel blockers (octanol and carbenoxolone), but not VRAC (fluoxetine) or pannexin (probenecid) channel blockers, inhibited fibroblast expansion. Together, these results support a mechanism in which extracellular ATP signaling via connexin hemichannels mediate the active change in fibroblast shape that occurs in response to a static increase in tissue length.

Langevin, Helene M.
Fujita, Takumi
Bouffard, Nicole A.
Takano, Takahiro
Koptiuch, Cathryn
Badger, Gary J.
Nedergaard, Maiken
Item Type: 
Journal Article
Publication Title: 
Journal of Cellular Physiology
Journal Abbreviation: 
J. Cell. Physiol.
Publication Date: 
Sep 2013
Publication Year: 
Library Catalog: 
NCBI Published Medical (?)
PMID: 23460361 PMCID: PMC3874839

Turabian/Chicago Citation

Helene M. Langevin, Takumi Fujita, Nicole A. Bouffard, Takahiro Takano, Cathryn Koptiuch, Gary J. Badger and Maiken Nedergaard. Sep 2013. "Fibroblast cytoskeletal remodeling induced by tissue stretch involves ATP signaling." Journal of Cellular Physiology 228: 9: 1922-1926. 10.1002/jcp.24356.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1002/jcp.24356 | issn = 1097-4652 | volume = 228 | pages = 1922-1926 | last = Langevin | first = Helene M. | coauthors = Fujita, Takumi, Bouffard, Nicole A., Takano, Takahiro, Koptiuch, Cathryn, Badger, Gary J., Nedergaard, Maiken | title = Fibroblast cytoskeletal remodeling induced by tissue stretch involves ATP signaling | journal = Journal of Cellular Physiology | date = Sep 2013 | pmid = | pmc = }} </ref>