Green tea extract modulates actin remodeling via Rho activity in an in vitro multistep carcinogenic model
Language: 
English
Abstract: 

Alteration of actin polymerization and loss of actin filaments is a marker of cellular dedifferentiation and early malignant transformation. To study this phenomenon, an in vitro human urothelial model consisting of two cell lines, HUC-PC and MC-T11, were incorporated into the study design. These two cell lines have different malignant transformation potential. The effect of green tea extract (GTE), a potential anticancer agent, on actin remodeling was investigated. Upon exposure to the carcinogen 4-aminobiphenyl (4-ABP), the untransformed HUC-PC undergoes malignant transformation whereas the transformed MC-T11 progresses from noninvasive to invasive tumor. GTE induces actin polymerization in MC-T11 cells in a dose-responsive manner, but this effect is less obvious in the untransformed, more differentiated HUC-PC cells, which natively have higher actin polymerization status. In contrast, GTE antagonizes carcinogen 4-ABP induced actin depolymerization and stress fiber disruption in HUC-PC cells. In MC-T11 cells, GTE inhibits 4-ABP induced motility by increasing cell adhesion and focal adhesion complex formation. The effect of GTE on actin remodeling seems to be mediated by the stimulation of small GTP-binding protein Rho activity, because C3 exoenzyme, a specific inhibitor for Rho, blocks GTE-mediated Rho activation and stress fiber formation in MC-T11 cells. This study shows that GTE exerts an effect on cytoskeletal actin remodeling and provides further support for the use of GTE as a chemopreventive agent.

Author(s): 
Lu, Qing-Yi
Jin, Yu-Sheng
Pantuck, Allan
Zhang, Zuo-Feng
Heber, David
Belldegrun, Arie
Brooks, Mai
Figlin, Robert
Rao, Jianyu
Item Type: 
Journal Article
Publication Title: 
Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Journal Abbreviation: 
Clin. Cancer Res.
Publication Date: 
Feb 15, 2005
Publication Year: 
2005
Pages: 
1675-1683
Volume: 
11
Issue: 
4
ISSN: 
1078-0432
DOI: 
10.1158/1078-0432.CCR-04-1608
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 15746073

Turabian/Chicago Citation

Qing-Yi Lu, Yu-Sheng Jin, Allan Pantuck, Zuo-Feng Zhang, David Heber, Arie Belldegrun, Mai Brooks, Robert Figlin and Jianyu Rao. Feb 15, 2005. "Green tea extract modulates actin remodeling via Rho activity in an in vitro multistep carcinogenic model." Clinical Cancer Research: An Official Journal of the American Association for Cancer Research 11: 4: 1675-1683. 10.1158/1078-0432.CCR-04-1608.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1158/1078-0432.CCR-04-1608 | issn = 1078-0432 | volume = 11 | pages = 1675-1683 | last = Lu | first = Qing-Yi | coauthors = Jin, Yu-Sheng, Pantuck, Allan, Zhang, Zuo-Feng, Heber, David, Belldegrun, Arie, Brooks, Mai, Figlin, Robert, Rao, Jianyu | title = Green tea extract modulates actin remodeling via Rho activity in an in vitro multistep carcinogenic model | journal = Clinical Cancer Research: An Official Journal of the American Association for Cancer Research | date = Feb 15, 2005 | pmid = | pmc = }} </ref>