Growth rate of late passage sarcoma cells is independent of epigenetic events but dependent on the amount of chromosomal aberrations
Language: 
English
Abstract: 

Soft tissue sarcomas (STS) are characterized by co-participation of several epigenetic and genetic events during tumorigenesis. Having bypassed cellular senescence barriers during oncogenic transformation, the factors further affecting growth rate of STS cells remain poorly understood. Therefore, we investigated the role of gene silencing (DNA promoter methylation of LINE-1, PTEN), genetic aberrations (karyotype, KRAS and BRAF mutations) as well as their contribution to the proliferation rate and migratory potential that underlies "initial" and "final" passage sarcoma cells. Three different cell lines were used, SW982 (synovial sarcoma), U2197 (malignant fibrous histiocytoma (MFH)) and HT1080 (fibrosarcoma). Increased proliferative potential of final passage STS cells was not associated with significant differences in methylation (LINE-1, PTEN) and mutation status (KRAS, BRAF), but it was dependent on the amount of chromosomal aberrations. Collectively, our data demonstrate that these fairly differentiated/advanced cancer cell lines have still the potential to gain an additional spontaneous growth benefit without external influences and that maintenance of increased proliferative potential towards longevity of STS cells (having crossed senescence barriers) may be independent of overt epigenetic alterations.

Author(s): 
Becerikli, Mustafa
Jacobsen, Frank
Rittig, Andrea
Kˆhne, Wiebke
Nambiar, Sandeep
Mirmohammadsadegh, Alireza
Stricker, Ingo
Tannapfel, Andrea
Wieczorek, Stefan
Epplen, Joerg Thomas
Tilkorn, Daniel
Steinstraesser, Lars
Item Type: 
Journal Article
Publication Title: 
Experimental Cell Research
Journal Abbreviation: 
Exp. Cell Res.
Publication Date: 
7/15/2013
Publication Year: 
2013
Pages: 
1724-1731
Volume: 
319
Issue: 
12
ISSN: 
1090-2422
DOI: 
10.1016/j.yexcr.2013.03.023
Library Catalog: 
NCBI Published Medical (?)
Extra: 
PMID: 23535008

Turabian/Chicago Citation

Mustafa Becerikli, Frank Jacobsen, Andrea Rittig, Wiebke Kˆhne, Sandeep Nambiar, Alireza Mirmohammadsadegh, Ingo Stricker, Andrea Tannapfel, Stefan Wieczorek, Joerg Thomas Epplen, Daniel Tilkorn and Lars Steinstraesser. 7/15/2013. "Growth rate of late passage sarcoma cells is independent of epigenetic events but dependent on the amount of chromosomal aberrations." Experimental Cell Research 319: 12: 1724-1731. 10.1016/j.yexcr.2013.03.023.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.yexcr.2013.03.023 | issn = 1090-2422 | volume = 319 | pages = 1724-1731 | last = Becerikli | first = Mustafa | coauthors = Jacobsen, Frank, Rittig, Andrea, Kˆhne, Wiebke, Nambiar, Sandeep, Mirmohammadsadegh, Alireza, Stricker, Ingo, Tannapfel, Andrea, Wieczorek, Stefan, Epplen, Joerg Thomas, Tilkorn, Daniel, Steinstraesser, Lars | title = Growth rate of late passage sarcoma cells is independent of epigenetic events but dependent on the amount of chromosomal aberrations | journal = Experimental Cell Research | date = 7/15/2013 | pmid = | pmc = }} </ref>