Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant
Language: 
English
Abstract: 

Our interest in development of hyaluronidase inhibitors as male antifertility agents led to identification of Terminalia chebula (T. chebula) plant with hyaluronidase (HAase) inhibitory activity of human spermatozoa ( approximately 93% inhibition) and rat caudal epididymal spermatozoa ( approximately 86% inhibition) in vitro at 30 mg/ml. We further demonstrated inhibition of hyaluronidase activity of testis and epididymal spermatozoa in vivo coincident with antispermatogenic activity and contraceptive efficacy of TC extract administered at 50 and 100mg/kg/day orally for 60 days in male albino rats. The significant decrease in motility, count and increase in morphological abnormalities of epididymal spermatozoa and severe reduction in fertility (-100%) of male rats treated with T. chebula fruit extract at 100mg/kg dose could be attributed to either direct effect on testis or direct or indirect interference with sperm maturation in epididymis, and/or inhibition of testicular and epididymal sperm hyaluronidase enzyme in vivo probably caused by flavonoids like tannins present in T. chebula.

Author(s): 
Srivastav, Archana
Chandra, Abhishek
Singh, Madhulika
Jamal, Farrukh
Rastogi, Preeti
Rajendran, Siron Mani
Bansode, Falgun Wanganuji
Lakshmi, Vijai
Item Type: 
Journal Article
Publication Title: 
Reproductive Toxicology (Elmsford, N.Y.)
Journal Abbreviation: 
Reprod. Toxicol.
Publication Date: 
2010-04
Publication Year: 
2010
Pages: 
214-224
Volume: 
29
Issue: 
2
ISSN: 
1873-1708
DOI: 
10.1016/j.reprotox.2009.11.001
Library Catalog: 
PubMed
Extra: 
PMID: 19903524

Turabian/Chicago Citation

Archana Srivastav, Abhishek Chandra, Madhulika Singh, Farrukh Jamal, Preeti Rastogi, Siron Mani Rajendran, Falgun Wanganuji Bansode and Vijai Lakshmi. 2010-04. "Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant." Reproductive Toxicology (Elmsford, N.Y.) 29: 2: 214-224. 10.1016/j.reprotox.2009.11.001.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1016/j.reprotox.2009.11.001 | issn = 1873-1708 | volume = 29 | pages = 214-224 | last = Srivastav | first = Archana | coauthors = Chandra, Abhishek, Singh, Madhulika, Jamal, Farrukh, Rastogi, Preeti, Rajendran, Siron Mani, Bansode, Falgun Wanganuji, Lakshmi, Vijai | title = Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant | journal = Reproductive Toxicology (Elmsford, N.Y.) | date = 2010-04 | pmid = | pmc = }} </ref>