Molecular Analysis of Terminalia spp. Distributed in Thailand and Authentication of Crude Drugs from Terminalia Plants
Language: 
English
Abstract: 

Terminalia, a large genus of Combretaceae, is distributed in Tropical Asia, Africa, and America. Some Terminalia plants are used in folk medicine because they possess powerful medicinal properties. Dried fruits of Terminalia bellirica and Terminalia chebula are used as the main ingredient in Triphala, a famous polyherbal formulation in Ayurvedic medicine and Thai folk medicine, because of their laxative, detoxifying, and rejuvenating effects. To clarify the phylogenetic relationships of medicinal Terminalia species (T. bellirica, T. chebula, and T. catappa) and authenticate their crude drugs, "Samo" and Triphala, nucleotide sequencing alignments in the internal transcribed spacer one-two (ITS 1-2) regions of Terminalia plants collected in Thailand were performed. The amplified fragments of Terminalia species were approximately 800?bp in length. To compare these sequences and DDBJ registered data, a molecular phylogenetic tree was constructed. Phylogenetic analysis clearly separated the sequences into two groups: Asian Terminalia and African Terminalia with some exceptions. In the analyzed sequences, the length of the ITS1-5.8S-ITS2 region was 674?bp in T. chebula, and 677?bp in T. bellirica and T. catappa. Eighty-one single nucleotide polymorphisms (SNPs) and nine insertion-deletions (indels) were observed, and the nucleotide sequences of this region showed species-specific sequences. Based on these differences, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) were applied to identify medicinal Terminalia species. Moreover, the ARMS method was chosen for fingerprinting analysis of Samo crude drugs and Triphala formulations because it was a fast, cost-effective, and reproducible approach.

Author(s): 
Intharuksa, Aekkhaluck
Ando, Hirokazu
Miyake, Katsunori
Sirisa-Ard, Panee
Mikage, Masayuki
Sasaki, Yohei
Item Type: 
Journal Article
Publication Title: 
Biological & Pharmaceutical Bulletin
Journal Abbreviation: 
Biol. Pharm. Bull.
Publication Date: 
2016
Publication Year: 
2016
Pages: 
492-501
Volume: 
39
Issue: 
4
ISSN: 
1347-5215
DOI: 
10.1248/bpb.b15-00673
Library Catalog: 
PubMed
Extra: 
PMID: 27040622

Turabian/Chicago Citation

Aekkhaluck Intharuksa, Hirokazu Ando, Katsunori Miyake, Panee Sirisa-Ard, Masayuki Mikage and Yohei Sasaki. 2016. "Molecular Analysis of Terminalia spp. Distributed in Thailand and Authentication of Crude Drugs from Terminalia Plants." Biological & Pharmaceutical Bulletin 39: 4: 492-501. 10.1248/bpb.b15-00673.

Wikipedia Citation

<ref> {{Cite journal | doi = 10.1248/bpb.b15-00673 | issn = 1347-5215 | volume = 39 | pages = 492-501 | last = Intharuksa | first = Aekkhaluck | coauthors = Ando, Hirokazu, Miyake, Katsunori, Sirisa-Ard, Panee, Mikage, Masayuki, Sasaki, Yohei | title = Molecular Analysis of Terminalia spp. Distributed in Thailand and Authentication of Crude Drugs from Terminalia Plants | journal = Biological & Pharmaceutical Bulletin | date = 2016 | pmid = | pmc = }} </ref>