Cell Movement

Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

The redox-sensitive transcription factor NF-E2-related factor 2 (Nrf2) plays a key role in preserving a healthy endothelial phenotype and maintaining the functional integrity of the vasculature. Previous studies demonstrated that aging is associated with Nrf2 dysfunction in endothelial cells, which alters redox signaling and likely promotes the development of large vessel disease. Much less is known about the consequences of Nrf2 dysfunction at the level of the microcirculation.

Author(s): 
Valcarcel-Ares, M. Noa
Gautam, Tripti
Warrington, Junie P.
Bailey-Downs, Lora
Sosnowska, Danuta
de Cabo, Rafael
Losonczy, Gyorgy
Sonntag, William E.
Ungvari, Zoltan
Csiszar, Anna
Publication Title: 
FASEB journal: official publication of the Federation of American Societies for Experimental Biology

Beneficial microbes and probiotics show promise for the treatment of pediatric gastrointestinal diseases. However, basic mechanisms of probiosis are not well understood, and most investigations have been performed in germ-free or microbiome-depleted animals. We sought to functionally characterize probiotic-host interactions in the context of normal early development. Outbred CD1 neonatal mice were orally gavaged with one of two strains of human-derived Lactobacillus reuteri or an equal volume of vehicle.

Author(s): 
Preidis, Geoffrey A.
Saulnier, Delphine M.
Blutt, Sarah E.
Mistretta, Toni-Ann
Riehle, Kevin P.
Major, Angela M.
Venable, Susan F.
Finegold, Milton J.
Petrosino, Joseph F.
Conner, Margaret E.
Versalovic, James
Publication Title: 
PloS One

Inflammatory bowel disease (IBD), consisting of Crohn's disease and ulcerative colitis (UC), results in substantial morbidity and is difficult to treat. New strategies for adjunct therapies are needed. One candidate is the semi-essential amino acid, L-arginine (L-Arg), a complementary medicine purported to be an enhancer of immunity and vitality in the lay media.

Author(s): 
Coburn, Lori A.
Gong, Xue
Singh, Kshipra
Asim, Mohammad
Scull, Brooks P.
Allaman, Margaret M.
Williams, Christopher S.
Rosen, Michael J.
Washington, M. Kay
Barry, Daniel P.
Piazuelo, M. Blanca
Casero, Robert A.
Chaturvedi, Rupesh
Zhao, Zhongming
Wilson, Keith T.
Publication Title: 
Hepatology (Baltimore, Md.)

RNA-binding proteins (RBPs) play a major role in the control of messenger RNA (mRNA) turnover and translation rates. We examined the role of the RBP, human antigen R (HuR), during cholestatic liver injury and hepatic stellate cell (HSC) activation. HuR silencing attenuated fibrosis development in vivo after BDL, reducing liver damage, oxidative stress, inflammation, and collagen and alpha smooth muscle actin (α-SMA) expression. HuR expression increased in activated HSCs from bile duct ligation mice and during HSC activation in vitro, and HuR silencing markedly reduced HSC activation.

Author(s): 
Woodhoo, Ashwin
Iruarrizaga-Lejarreta, Marta
Beraza, Naiara
García-Rodríguez, Juan L.
Embade, Nieves
Fernández-Ramos, David
Martínez-López, Nuria
Gutierrez-de Juan, Virginia
Arteta, Beatriz
Caballeria, Juan
Lu, Shelly C.
Mato, José M.
Varela-Rey, Marta
Martínez-Chantar, María L.
Publication Title: 
Chinese Journal of Integrative Medicine

OBJECTIVE: To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction () on the endothelial cell line ECV304. METHODS: ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction-containing serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.

Author(s): 
Song, Jun
Chen, Wen-Yuan
Wu, Li-Ya
Zheng, Liang-Pu
Lin, Wei
Gao, Dong
Kaptchuk, Ted J.
Chen, Ke-ji
Publication Title: 
American Journal of Physiology. Lung Cellular and Molecular Physiology

Clinical reports indicate that patients with allergy/asthma commonly have associated symptoms of anxiety/depression. Anxiety/depression can be reduced by 5-hydroxytryptophan (5-HTP) supplementation. However, it is not known whether 5-HTP reduces allergic inflammation. Therefore, we determined whether 5-HTP supplementation reduces allergic inflammation. We also determined whether 5-HTP decreases passage of leukocytes through the endothelial barrier by regulating endothelial cell function.

Author(s): 
Abdala-Valencia, Hiam
Berdnikovs, Sergejs
McCary, Christine A.
Urick, Daniela
Mahadevia, Riti
Marchese, Michelle E.
Swartz, Kelsey
Wright, Lakiea
Mutlu, Gökhan M.
Cook-Mills, Joan M.
Publication Title: 
Bioorganic & Medicinal Chemistry

Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the synovial joints, deformities, and disability. The prolonged use of conventional anti-inflammatory drugs is associated with severe adverse effects. Therefore, there is an urgent need for safer and less expensive therapeutic products. Celastrol is a bioactive component of Celastrus, a traditional Chinese medicine, and it possesses anti-arthritic activity. However, the mechanism of action of Celastrol remains to be fully defined.

Author(s): 
Venkatesha, Shivaprasad H.
Astry, Brian
Nanjundaiah, Siddaraju M.
Yu, Hua
Moudgil, Kamal D.
Publication Title: 
Pancreas

OBJECTIVES: Tobacco-derived carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) activates β-adrenergic receptor (β-AR) signaling through Src/focal adhesion kinases (FAKs)/mitogen-activated protein kinase to modulate proliferation, migration, and survival. Apigenin (4', 5, 7-trihydroxyflavone) is reported to attenuate proliferation and migration of cancer cells. This study was designed to determine the effects of apigenin on NNK-induced procarcinogenesis using human pancreatic cancer cells BxPC-3 and MIA PaCa-2, which express β-AR.

Author(s): 
Pham, Hung
Chen, Monica
Takahashi, Hiroki
King, Jonathan
Reber, Howard A.
Hines, Oscar Joe
Pandol, Stephen
Eibl, Guido
Publication Title: 
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences

Moderate caloric restriction (CR) without malnutrition increases healthspan in virtually every species studied, including nonhuman primates. In mice, CR exerts significant microvascular protective effects resulting in increased microvascular density in the heart and the brain, which likely contribute to enhanced tolerance to ischemia and improved cardiac performance and cognitive function. Yet, the underlying mechanisms by which CR confer microvascular protection remain elusive.

Author(s): 
Csiszar, Anna
Sosnowska, Danuta
Tucsek, Zsuzsanna
Gautam, Tripti
Toth, Peter
Losonczy, Gyorgy
Colman, Ricki J.
Weindruch, Richard
Anderson, Rozalyn M.
Sonntag, William E.
Ungvari, Zoltan
Publication Title: 
Biochemistry

Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for leukocyte transendothelial migration on VCAM-1. However, it has not been reported whether the cytoplasmic domain of VCAM-1 is necessary for these signals.

Author(s): 
Marchese, Michelle E.
Berdnikovs, Sergejs
Cook-Mills, Joan M.

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