Cytoskeletal Proteins

Publication Title: 
PloS One

BACKGROUND: In a variety of organisms, including mammals, caloric restriction improves metabolic status and lowers the incidence of chronic-degenerative diseases, ultimately leading to increased lifespan. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that knockout mice for Eps8, a regulator of actin dynamics, display reduced body weight, partial resistance to age- or diet-induced obesity, and overall improved metabolic status. Alteration in the liver gene expression profile, in behavior and metabolism point to a calorie restriction-like phenotype in Eps8 knockout mice.

Author(s): 
Tocchetti, Arianna
Soppo, Charlotte Blanche Ekalle
Zani, Fabio
Bianchi, Fabrizio
Gagliani, Maria Cristina
Pozzi, Benedetta
Rozman, Jan
Elvert, Ralf
Ehrhardt, Nicole
Rathkolb, Birgit
Moerth, Corinna
Horsch, Marion
Fuchs, Helmut
Gailus-Durner, ValÈrie
Beckers, Johannes
Klingenspor, Martin
Wolf, Eckhard
HrabÈ de Angelis, Martin
Scanziani, Eugenio
Tacchetti, Carlo
Scita, Giorgio
Di Fiore, Pier Paolo
Offenh‰user, Nina
Publication Title: 
Cellular and molecular life sciences: CMLS

Alcohol use and abuse appear to be related to neuroadaptive changes at functional, neurochemical, and structural levels. Acute and chronic ethanol exposure have been shown to modulate function of the activity-dependent gene transcription factor, cAMP-responsive element binding (CREB) protein in the brain, which may be associated with the development of alcoholism.

Author(s): 
Moonat, Sachin
Starkman, Bela G.
Sakharkar, Amul
Pandey, Subhash C.
Publication Title: 
PloS One

The PCLO rs2522833 candidate polymorphism for depression has been associated to monoaminergic neurotransmission. In healthy and currently depressed individuals, the polymorphism has been found to affect activation of brain areas during memory processing, but no direct association of PCLO with memory bias was found. We hypothesized that the absence of this association might have been obscured by current depressive symptoms or genetically driven individual differences in reactivity to stressful events.

Author(s): 
Vrijsen, Janna N.
Speckens, Anne
Arias-V·squez, Alejandro
Franke, Barbara
Becker, Eni S.
van Oostrom, Iris
Publication Title: 
The Journal of Biological Chemistry

Osteoblast lineage-specific differentiation of mesenchymal stem cells is a well regulated but poorly understood process. Both bone morphogenetic proteins (BMPs) and Wnt signaling are implicated in regulating osteoblast differentiation and bone formation. Here we analyzed the expression profiles of mesenchymal stem cells stimulated with Wnt3A and osteogenic BMPs, and we identified connective tissue growth factor (CTGF) as a potential target of Wnt and BMP signaling.

Author(s): 
Luo, Qing
Kang, Quan
Si, Weike
Jiang, Wei
Park, Jong Kyung
Peng, Ying
Li, Xinmin
Luu, Hue H.
Luo, Jeffrey
Montag, Anthony G.
Haydon, Rex C.
He, Tong-Chuan
Publication Title: 
American Journal of Physiology. Heart and Circulatory Physiology

Hyperglycemia in diabetes mellitus promotes oxidative stress in endothelial cells, which contributes to development of cardiovascular diseases. Nuclear factor erythroid 2-related factor-2 (Nrf2) is a transcription factor activated by oxidative stress that regulates expression of numerous reactive oxygen species (ROS) detoxifying and antioxidant genes. This study was designed to elucidate the homeostatic role of adaptive induction of Nrf2-driven free radical detoxification mechanisms in endothelial protection under diabetic conditions.

Author(s): 
Ungvari, Zoltan
Bailey-Downs, Lora
Gautam, Tripti
Jimenez, Rosario
Losonczy, Gyorgy
Zhang, Cuihua
Ballabh, Praveen
Recchia, Fabio A.
Wilkerson, Donald C.
Sonntag, William E.
Pearson, Kevin
de Cabo, Rafael
Csiszar, Anna
Publication Title: 
Stroke; a Journal of Cerebral Circulation

BACKGROUND AND PURPOSE: Carbon monoxide (CO) is a gaseous second messenger produced when heme oxygenase enzymes catabolize heme. We have demonstrated that CO can be therapeutic in ischemia-reperfusion brain injury; however, it is unclear whether CO can also offer protection in permanent ischemic stroke or what mechanism(s) underlies the effect. Heme oxygenase-1 neuroprotection was shown to be regulated by Nrf2; therefore, we investigated whether CO might partially exert neuroprotection by modulating the Nrf2 pathway.

Author(s): 
Wang, Bing
Cao, Wangsen
Biswal, Shyam
Doré, Sylvain
Publication Title: 
Pharmaceutical Research

PURPOSE: CR increases fatty acid oxidation to decrease tissue lipid content. The Nuclear factor E2-related factor 2 (Nrf2)-Kelch like ECH associated Protein 1 (Keap1) pathway is an antioxidant gene regulatory pathway that has been previously investigated in weight gain. However, limited interaction of Nrf2/Keap1 and CR exists. The purpose of this study was to determine how Keap1 knockdown (Keap1-KD), which is known to increase Nrf2 activity, affects the CR response, such as weight loss, hepatic lipid decrease, and induction of fatty acid oxidation gene expression.

Author(s): 
Kulkarni, Supriya R.
Armstrong, Laura E.
Slitt, Angela L.
Publication Title: 
Life Sciences

AIMS: Centella asiatica has been used to treat kidney diseases in Chinese traditional medicine. Asiaticoside (an extraction of C. asiatica) exerts a variety of pharmacological effects including immunomodulatory and anti-inflammatory functions. However, the mechanism of asiaticoside in the treatment of renal diseases remains largely unknown. This study investigated the molecular mechanism of asiaticoside in treating adriamycin-induced nephropathy of rats. MAIN METHODS: Sixty-two SD male rats were randomly divided into normal control group (n=12) and nephropathy group (n=50).

Author(s): 
Wang, Zhu
Liu, Juntian
Sun, Wansen
Subscribe to RSS - Cytoskeletal Proteins