Enzymes, Immobilized

Publication Title: 
Biotechnology Letters

Theaflavins were synthesized from tea polyphenols extracted from green tea using an immobilized polyphenol oxidase system. To optimize the production of theaflavins, response surface methodology was applied to determine the effects of five critical variables and their mutual interactions on theaflavin biosynthesis at five levels. A total of 52 individual experiments were performed and a statistical model predicted that the highest theaflavin concentration was 0.766 mg ml(-1) at optimized conditions.

Author(s): 
Tu, You-ying
Xu, Xin-Qing
Xia, Hui-Long
Watanabe, Naoharu
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