PURPOSE: To investigate the migratory and contractile behavior of isolated human corneal fibroblasts in fibrillar collagen matrices. METHODS: A telomerase-infected, extended-lifespan human corneal fibroblast cell line (HTK) was transfected by using a vector for enhanced green fluorescent protein (GFP)-alpha-actinin. Cells were plated at low density on top of or within 100-microm-thick fibrillar collagen lattices. After 18 hours to 7 days, time-lapse imaging was performed.
Journal of Manipulative and Physiological Therapeutics
OBJECTIVE: The purpose of this study was to quantify the biomechanical changes that occur in a compressed cervical disk with the application of axial distraction when the annular fiber orientation angles are varied between the horizontal and vertical planes. METHODS: A 3-dimensional finite element (FE) model of a cervical motion segment was developed.