Genetic Markers

Publication Title: 
Brazilian Journal of Biology = Revista Brasleira De Biologia

Maytenus ilicifolia is a medicinal plant largely used in the South Brazilian folk medicine. The aim of this study was to quantify the intra and inter populational genetic variability in three populations of M. ilicifolia, focusing on the genetic conservation of this species, which has been threatened by anthropic action. RAPD (Random Amplified Polymorphic DNA) markers were used to analyze 30 plants of each of the three populations collected in the Alto Uruguai Gaúcho region. Fourteen selected primers generated a total of 158 bands, 71.5% of which were polymorphic.

Author(s): 
Mossi, A. J.
Cansian, R. L.
Leontiev-Orlov, O.
Zanin, E. M.
Oliveira, C. H.
Cechet, M. L.
Carvalho, A. Z.
Echeverrigaray, S.
Publication Title: 
Journal of Animal Science

Escalating replacement rates and production costs warrant attention on sow productive life (SPL). Increasing average SPL by one-tenth of 1 parity would result in an annual revenue increase of over $15 million in the United States. Research in model organisms has revealed conserved genes and gene pathways that lead to longer lifespan. The most prominent gene pathways are those involved in growth, most notably genes in the IGF pathway that serve to mimic the response of caloric restriction.

Author(s): 
Mote, B. E.
Koehler, K. J.
Mabry, J. W.
Stalder, K. J.
Rothschild, M. F.
Publication Title: 
Acta Pharmacologica Sinica

AIM: An efficient, precise, and sensitive method for identifying Atractylodes plants has been established and will contribute significantly to quality control and scientific analysis in Chinese traditional medicine. METHODS: Twenty primers were applied for setting up the RAPD (randomly amplified polymorphic DNA) markers of Atractylodes plants, Atractylodes lancea DC (A lancea DC), Atractylodes japonica Koidz (A japonica K), and Atractylodes ovata DC (A ovata DC). The primer OPF03, OPF05, and OPF14 could discriminate them successfully.

Author(s): 
Chen, K. T.
Su, Y. C.
Lin, J. G.
Hsin, L. H.
Su, Y. P.
Su, C. H.
Li, S. Y.
Cheng, J. H.
Mao, S. J.
Publication Title: 
Biological & Pharmaceutical Bulletin

Medicinal pteridophytes are an important group used in traditional Chinese medicine; however, there is no simple and universal way to differentiate various species of this group by morphological traits. A novel technology termed "DNA barcoding" could discriminate species by a standard DNA sequence with universal primers and sufficient variation.

Author(s): 
Ma, Xin-Ye
Xie, Cai-Xiang
Liu, Chang
Song, Jing-Yuan
Yao, Hui
Luo, Kun
Zhu, Ying-jie
Gao, Ting
Pang, Xiao-Hui
Qian, Jun
Chen, Shi-Lin
Publication Title: 
Genetics and molecular research: GMR

Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellite loci from a (CT)(n)-enriched genomic library of P. polyphylla var. chinensis. The polymorphism at each locus was analyzed by screening 30 individuals from a natural population. The number of alleles ranged from 2 to 5.

Author(s): 
Zheng, J. Y.
Wang, H.
Chen, X. X.
Wang, P.
Gao, P.
Li, X. N.
Zhu, G. P.
Publication Title: 
Genetics and molecular research: GMR

Dendrobium officinale is an endangered orchid from southeast Asia that is known for its medicinal properties in traditional Chinese medicine. We constructed an integrated genetic linkage map of an F(1) population derived from an interspecific cross between D. officinale and D. aduncum (both, 2n = 38), using expressed sequence tag-simple sequence repeats (EST-SSR) and sequence-related amplified polymorphism (SRAP). A total of 349 polymorphic loci, including 261 SRAP loci and 88 EST-SSR loci, were identified for genetic linkage analysis.

Author(s): 
Lu, J. J.
Wang, S.
Zhao, H. Y.
Liu, J. J.
Wang, H. Z.
Publication Title: 
Diabetologia

AIMS/HYPOTHESIS: Most genetic variants identified for type 2 diabetes have been discovered in European populations. We performed genome-wide association studies (GWAS) in a Chinese population with the aim of identifying novel variants for type 2 diabetes in Asians. METHODS: We performed a meta-analysis of three GWAS comprising 684 patients with type 2 diabetes and 955 controls of Southern Han Chinese descent.

Author(s): 
Ma, R. C. W.
Hu, C.
Tam, C. H.
Zhang, R.
Kwan, P.
Leung, T. F.
Thomas, G. N.
Go, M. J.
Hara, K.
Sim, X.
Ho, J. S. K.
Wang, C.
Li, H.
Lu, L.
Wang, Y.
Li, J. W.
Wang, Y.
Lam, V. K. L.
Wang, J.
Yu, W.
Kim, Y. J.
Ng, D. P.
Fujita, H.
Panoutsopoulou, K.
Day-Williams, A. G.
Lee, H. M.
Ng, A. C. W.
Fang, Y.-J.
Kong, A. P. S.
Jiang, F.
Ma, X.
Hou, X.
Tang, S.
Lu, J.
Yamauchi, T.
Tsui, S. K. W.
Woo, J.
Leung, P. C.
Zhang, X.
Tang, N. L. S.
Sy, H. Y.
Liu, J.
Wong, T. Y.
Lee, J. Y.
Maeda, S.
Xu, G.
Cherny, S. S.
Chan, T. F.
Ng, M. C. Y.
Xiang, K.
Morris, A. P.
DIAGRAM Consortium
Keildson, S.
MuTHER Consortium
Hu, R.
Ji, L.
Lin, X.
Cho, Y. S.
Kadowaki, T.
Tai, E. S.
Zeggini, E.
McCarthy, M. I.
Hon, K. L.
Baum, L.
Tomlinson, B.
So, W. Y.
Bao, Y.
Chan, J. C. N.
Jia, W.
Publication Title: 
Frontiers of Medicine

Lung cancer is the leading cause of cancer-related deaths in the US and worldwide. Better understanding of the disease is warranted for improvement in clinical management. Here we summarize the functions of small-RNA-based, posttranscriptional gene regulators, i.e. microRNAs, in the pathogenesis of lung cancers. We discuss the microRNAs that play oncogenic as well as tumor suppressive roles. We also touch on the value of microRNAs as markers for diagnosis, prognosis and the promising field of microRNA-based novel therapies for lung cancers.

Author(s): 
Qi, Ji
Mu, David
Publication Title: 
Biomedical and environmental sciences: BES

Parkinson's disease (PD) is a typical degenerative disease, which is characterized by the most obvious symptoms of movement dysfunction, including shaking, rigidity, slowness of movement and difficulty in walking and gait. This disease can not be clearly identified through laboratory tests at present, thus application of high-throughput technique in studying the expression profiles of PD helps to find the genetic markers for its early diagnosis. Studies on expression profiles of neurodegenerative diseases have revealed the novel genes and pathways involved in the progress of illness.

Author(s): 
Zhang, Yi
Yao, Li
Liu, Wei
Li, Wei
Tian, Chan
Wang, Zhao Yang
Liu, Di

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