Growth Differentiation Factor 2

Publication Title: 
The Journal of Biological Chemistry

Bone morphogenetic proteins (BMPs) belong to the TGF-beta superfamily and play an important role in development and in many cellular processes. We have found that BMP-2, BMP-6, and BMP-9 induce the most potent osteogenic differentiation of mesenchymal stem cells. Expression profiling analysis has revealed that the Inhibitors of DNA binding/differentiation (Id)-1, Id-2, and Id-3 are among the most significantly up-regulated genes upon BMP-2, BMP-6, or BMP-9 stimulation. Here, we sought to determine the functional role of these Id proteins in BMP-induced osteoblast differentiation.

Author(s): 
Peng, Ying
Kang, Quan
Luo, Qing
Jiang, Wei
Si, Weike
Liu, Bernard A.
Luu, Hue H.
Park, Jong Kyung
Li, Xinmin
Luo, Jeffrey
Montag, Anthony G.
Haydon, Rex C.
He, Tong-Chuan
Publication Title: 
The Journal of Biological Chemistry

Osteoblast lineage-specific differentiation of mesenchymal stem cells is a well regulated but poorly understood process. Both bone morphogenetic proteins (BMPs) and Wnt signaling are implicated in regulating osteoblast differentiation and bone formation. Here we analyzed the expression profiles of mesenchymal stem cells stimulated with Wnt3A and osteogenic BMPs, and we identified connective tissue growth factor (CTGF) as a potential target of Wnt and BMP signaling.

Author(s): 
Luo, Qing
Kang, Quan
Si, Weike
Jiang, Wei
Park, Jong Kyung
Peng, Ying
Li, Xinmin
Luu, Hue H.
Luo, Jeffrey
Montag, Anthony G.
Haydon, Rex C.
He, Tong-Chuan
Publication Title: 
Journal of Bone and Mineral Research: The Official Journal of the American Society for Bone and Mineral Research

Efficient osteogenic differentiation and bone formation from mesenchymal stem cells (MSCs) should have clinical applications in treating nonunion fracture healing. MSCs are adherent bone marrow stromal cells that can self-renew and differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. We have identified bone morphogenetic protein 9 (BMP-9) as one of the most osteogenic BMPs. Here we investigate the effect of insulin-like growth factor 2 (IGF-2) on BMP-9-induced bone formation. We have found that endogenous IGF-2 expression is low in MSCs.

Author(s): 
Chen, Liang
Jiang, Wei
Huang, Jiayi
He, Bai-Cheng
Zuo, Guo-Wei
Zhang, Wenli
Luo, Qing
Shi, Qiong
Zhang, Bing-Qiang
Wagner, Eric R.
Luo, Jinyong
Tang, Min
Wietholt, Christian
Luo, Xiaoji
Bi, Yang
Su, Yuxi
Liu, Bo
Kim, Stephanie H.
He, Connie J.
Hu, Yawen
Shen, Jikun
Rastegar, Farbod
Huang, Enyi
Gao, Yanhong
Gao, Jian-Li
Zhou, Jian-Zhong
Reid, Russell R.
Luu, Hue H.
Haydon, Rex C.
He, Tong-Chuan
Deng, Zhong-Liang
Publication Title: 
The Journal of Biological Chemistry

Mesenchymal stem cells (MSCs) are bone marrow stromal cells that can differentiate into multiple lineages. We previously demonstrated that BMP9 is one of the most potent BMPs to induce osteogenic differentiation of MSCs. BMP9 is one of the least studied BMPs. Whereas ALK1, ALK5, and/or endoglin have recently been reported as potential BMP9 type I receptors in endothelial cells, little is known about type I receptor involvement in BMP9-induced osteogenic differentiation in MSCs.

Author(s): 
Luo, Jinyong
Tang, Min
Huang, Jiayi
He, Bai-Cheng
Gao, Jian-Li
Chen, Liang
Zuo, Guo-Wei
Zhang, Wenli
Luo, Qing
Shi, Qiong
Zhang, Bing-Qiang
Bi, Yang
Luo, Xiaoji
Jiang, Wei
Su, Yuxi
Shen, Jikun
Kim, Stephanie H.
Huang, Enyi
Gao, Yanhong
Zhou, Jian-Zhong
Yang, Ke
Luu, Hue H.
Pan, Xiaochuan
Haydon, Rex C.
Deng, Zhong-Liang
He, Tong-Chuan
Publication Title: 
PloS One

BACKGROUND: As one of the least studied bone morphogenetic proteins (BMPs), BMP9 is one of the most osteogenic BMPs. Retinoic acid (RA) signaling is known to play an important role in development, differentiation and bone metabolism. In this study, we investigate the effect of RA signaling on BMP9-induced osteogenic differentiation of mesenchymal progenitor cells (MPCs). METHODOLOGY/PRINCIPAL FINDINGS: Both primary MPCs and MPC line are used for BMP9 and RA stimulation. Recombinant adenoviruses are used to deliver BMP9, RARalpha and RXRalpha into MPCs.

Author(s): 
Zhang, Wenli
Deng, Zhong-Liang
Chen, Liang
Zuo, Guo-Wei
Luo, Qing
Shi, Qiong
Zhang, Bing-Qiang
Wagner, Eric R.
Rastegar, Farbod
Kim, Stephanie H.
Jiang, Wei
Shen, Jikun
Huang, Enyi
Gao, Yanhong
Gao, Jian-Li
Zhou, Jian-Zhong
Luo, Jinyong
Huang, Jiayi
Luo, Xiaoji
Bi, Yang
Su, Yuxi
Yang, Ke
Liu, Hao
Luu, Hue H.
Haydon, Rex C.
He, Tong-Chuan
He, Bai-Cheng
Publication Title: 
Journal of Cell Science

Mesenchymal stromal progenitor cells (MSCs) are multipotent progenitors that can be isolated from numerous tissues. MSCs can undergo osteogenic differentiation under proper stimuli. We have recently demonstrated that bone morphogenetic protein 9 (BMP9) is one of the most osteogenic BMPs. As one of the least studied BMPs, BMP9 has been shown to regulate angiogenesis in endothelial cells. However, it is unclear whether BMP9-regulated angiogenic signaling plays any important role in the BMP9-initiated osteogenic pathway in MSCs.

Author(s): 
Hu, Ning
Jiang, Dianming
Huang, Enyi
Liu, Xing
Li, Ruidong
Liang, Xi
Kim, Stephanie H.
Chen, Xiang
Gao, Jian-Li
Zhang, Hongyu
Zhang, Wenwen
Kong, Yu-Han
Zhang, Jiye
Wang, Jinhua
Shui, Wei
Luo, Xiaoji
Liu, Bo
Cui, Jing
Rogers, Mary Rose
Shen, Jikun
Zhao, Chen
Wang, Ning
Wu, Ningning
Luu, Hue H.
Haydon, Rex C.
He, Tong-Chuan
Huang, Wei
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