Ion Transport

Publication Title: 
Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology

Little is known about the relationship between CF transmembrane conductance regulator (CFTR) gene expression and the corresponding transport of Cl. The phenotypic characteristics of polarized DeltaF508 homozygote CF bronchial epithelial (CFBE41o-) cells were evaluated following transfection with episomal expression vector containing either full-length (6.2kb) wild type (wt) and (4.7kb) DeltaF508CFTR cDNA.

Author(s): 
Illek, Beate
Maurisse, Rosalie
Wahler, Logan
Kunzelmann, Karl
Fischer, Horst
Gruenert, Dieter C.
Publication Title: 
Free Radical Biology & Medicine

Pyocyanin (N-methyl-1-hydroxyphenazine), a redox-active virulence factor produced by the human pathogen Pseudomonas aeruginosa, is known to compromise mucociliary clearance. Exposure of human bronchial epithelial cells to pyocyanin increased the rate of cellular release of H(2)O(2) threefold above the endogenous H(2)O(2) production.

Author(s): 
Schwarzer, Christian
Fischer, Horst
Kim, Eun-Jin
Barber, Katharine J.
Mills, Aaron D.
Kurth, Mark J.
Gruenert, Dieter C.
Suh, Jung H.
Machen, Terry E.
Illek, Beate
Publication Title: 
American Journal of Rhinology & Allergy

BACKGROUND: Chronic rhinosinusitis (CRS) occurs at high frequency in patients with cystic fibrosis, suggesting that the cystic fibrosis transmembrane conductance regulator (CFTR) chloride (Cl) ion channel might be involved in the development of chronic sinusitis in the general population. CFTR Cl ion transport controls the hydration of mucosal surfaces and promotes effective mucociliary clearance. Altered ion transport and, hence, disrupted mucociliary function, could play a role in the pathogenesis of sinus disease.

Author(s): 
Cho, Do-Yeon
Hwang, Peter H.
Illek, Beate
Publication Title: 
American Journal of Rhinology & Allergy

BACKGROUND: Proton (H+) secretion and the HVCN1 H+ channel are part of the innate host defense mechanism of the airways. The objective of this study was to determine H+ secretion in asthmatic and nonasthmatic patients with chronic rhinosinusitis (CRS) in freshly excised human sinonasal tissue. METHODS: Nasal or sinus mucosa from subjects with three different conditions (normal, CRS, and CRS with asthma) was harvested during sinus surgery. The equilibrium pH and the rate of H+ secretion were measured in an Ussing chamber using the pH-stat titration technique.

Author(s): 
Cho, Do-Yeon
Hajighasemi, Mohammad
Hwang, Peter H.
Illek, Beate
Fischer, Horst
Publication Title: 
American Journal of Respiratory Cell and Molecular Biology

Therapies to correct the ΔF508 cystic fibrosis transmembrane conductance regulator (CFTR) folding defect require sensitive methods to detect channel activity in vivo. The β₂ adrenergic receptor agonists, which provide the CFTR stimuli commonly used in nasal potential difference assays, may not overcome the channel gating defects seen in ΔF508 CFTR after plasma membrane localization. In this study, we identify an agent, quercetin, that enhances the detection of surface ΔF508 CFTR, and is suitable for nasal perfusion.

Author(s): 
Pyle, Louise C.
Fulton, Jennifer C.
Sloane, Peter A.
Backer, Kyle
Mazur, Marina
Prasain, Jeevan
Barnes, Stephen
Clancy, J. P.
Rowe, Steven M.
Publication Title: 
Toxicological Sciences: An Official Journal of the Society of Toxicology

Maintenance of ionic balance is essential for neuronal functioning. Hydrogen sulfide (H(2)S), a known toxic environmental gaseous pollutant, has been recently recognized as a gasotransmitter involved in numerous biological processes and is believed to play an important role in the neural activities under both physiological and pathological conditions. However, it is unclear if it plays any role in maintenance of ionic homeostasis in the brain under physiological/pathophysiological conditions.

Author(s): 
Chao, Dongman
He, Xiaozhou
Yang, Yilin
Balboni, Gianfranco
Salvadori, Severo
Kim, Dong H.
Xia, Ying
Publication Title: 
Biochimica Et Biophysica Acta

BACKGROUND: Herein we report the discovery of a cystine-crosslinked peptide from Porifera along with high-quality spatial details accompanied by the description of its unique effect on neuronal calcium influx. METHODS: Asteropsin A (ASPA) was isolated from the marine sponge Asteropus sp., and its structure was independently determined using X-ray crystallography (0.87 angstroms) and solution NMR spectroscopy.

Author(s): 
Li, Huayue
Bowling, John J.
Fronczek, Frank R.
Hong, Jongki
Jabba, Sairam V.
Murray, Thomas F.
Ha, Nam-Chul
Hamann, Mark T.
Jung, Jee H.
Publication Title: 
Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences

Short circuit current (I(sc)) technique has been applied in the studies of transepithelial ion transport in various epithelia. Recently it has also been used in the modernization researches on traditional Chinese medicines. This review gives an overview of the basic principle of the I(sc) technique, the targets of measurement, ion transport, ion channel, the general ways of I(sc) research design, the application of I(sc) technology in the researches on traditional Chinese medicines.

Author(s): 
Yuan, Jin-song
Li, Kang
Yang, Da-jian
Sui, Li-ying
Liu, Yan
Zhang, Chao
Guo, Chang-jie
Subscribe to RSS - Ion Transport