Microsatellite Repeats

Publication Title: 
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Inheritance and linkage studies were carried out with microsatellite [or simple sequence repeat (SSR)] markers in a F(1) progeny including 101 individuals of a cross between Myrobalan plum ( Prunus cerasifera Ehrh) clone P.2175 and the almond (Prunus dulcis Mill.)-peach ( Prunus persica L. Batsch) hybrid clone GN22 ["Garfi" (G) almond x "Nemared" (N) peach].

Author(s): 
Dirlewanger, E.
Cosson, P.
Howad, W.
Capdeville, G.
Bosselut, N.
Claverie, M.
Voisin, R.
Poizat, C.
Lafargue, B.
Baron, O.
Laigret, F.
Kleinhentz, M.
Arús, P.
Esmenjaud, D.
Publication Title: 
Molecular Biology Reports

Terminalia trees are being over-exploited because of their medicinal and economical importance leading to loss of valuable genetic resources. For sustainable utilization and conservation, assessment of genetic diversity therefore becomes imperative. We report a comprehensive first study on estimation and analysis of genetic variation through Amplified fragment length polymorphism (AFLP), inter simple sequence repeat polymorphism (ISSR) and random amplification of polymorphic DNA (RAPD) across three species of Terminalia.

Author(s): 
Sarwat, Maryam
Das, Sandip
Srivastava, Prem S.
Publication Title: 
The American Journal of Psychiatry

OBJECTIVE: Some genome-wide scans and association studies for schizophrenia susceptibility genes have yielded significant positive findings, but there is disagreement between studies on their locations, and no mutation has yet been found in any gene. Since schizophrenia is a complex disorder, a study with sufficient power to detect a locus with a small or moderate gene effect is necessary.

Author(s): 
DeLisi, Lynn E.
Shaw, Sarah H.
Crow, Timothy J.
Shields, Gail
Smith, Angela B.
Larach, Veronica W.
Wellman, Nigel
Loftus, Josephine
Nanthakumar, Betsy
Razi, Kamran
Stewart, John
Comazzi, Margherita
Vita, Antonio
Heffner, Thomas
Sherrington, Robin
Publication Title: 
Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases

Human travel to malaria endemic lowlands from epidemic highlands has been shown to increase the risk of malaria infections in the highlands. In order to gain insight on the impact of human travel, we examined prevalence, genetic variability and population genetic structure of Plasmodium falciparum in asymptomatic children from one highland site and three surrounding malaria endemic lowland sites in Western Kenya, using multilocus microsatellite genotyping. We further analyzed the frequencies of mutations at the genes conferring resistance to chloroquine and sulfadoxine-pyrimethamine.

Author(s): 
Bonizzoni, Mariangela
Afrane, Yaw
Baliraine, Frederick N.
Amenya, Dolphine A.
Githeko, Andrew K.
Yan, Guiyun
Publication Title: 
The Journal of Infectious Diseases

In western Cambodia, malaria parasites clear slowly from the blood after treatment with artemisinin derivatives, but it is unclear whether this results from parasite, host, or other factors specific to this population. We measured heritability of clearance rate by evaluating patients infected with identical or nonidentical parasite genotypes, using methods analogous to human twin studies. A substantial proportion (56%-58%) of the variation in clearance rate is explained by parasite genetics.

Author(s): 
Anderson, Tim J. C.
Nair, Shalini
Nkhoma, Standwell
Williams, Jeff T.
Imwong, Mallika
Yi, Poravuth
Socheat, Duong
Das, Debashish
Chotivanich, Kesinee
Day, Nicholas P. J.
White, Nicholas J.
Dondorp, Arjen M.
Publication Title: 
The Journal of Infectious Diseases

BACKGROUND: The emergence of artesunate-mefloquine (AS+MQ)-resistant Plasmodium falciparum in the Thailand-Cambodia region is a major concern for malaria control. Studies indicate that copy number increase and key alleles in the pfmdr1 gene are associated with AS+MQ resistance. In the present study, we investigated evidence for a selective sweep around pfmdr1 because of the spread of adaptive mutation and/or multiple copies of this gene in the P. falciparum population in Cambodia. METHODS: We characterized 13 microsatellite loci flanking (+/-99 kb) pfmdr1 in 93 single-clone P.

Author(s): 
Vinayak, Sumiti
Alam, Md Tauqeer
Sem, Rithy
Shah, Naman K.
Susanti, Augustina I.
Lim, Pharath
Muth, Sinuon
Maguire, Jason D.
Rogers, William O.
Fandeur, Thierry
Barnwell, John W.
Escalante, Ananias A.
Wongsrichanalai, Chansuda
Ariey, Frederick
Meshnick, Steven R.
Udhayakumar, Venkatachalam
Publication Title: 
The Journal of Infectious Diseases

BACKGROUND: In 2005, Ghana adopted artemisinin-based combination therapy (ACT) for primary treatment of falciparum malaria. A comprehensive study of the drug-resistance-associated mutations and their genetic lineages will lead to a better understanding of the evolution of antimalarial drug resistance in this region. METHODS: The pfcrt, pfmdr1, dhps, and dhfr mutations associated with chloroquine (CQ) and sulfadoxine-pyrimethamine (SP) resistance and the microsatellite loci flanking these genes were genotyped in Plasmodium falciparum isolates from Ghana.

Author(s): 
Alam, Md Tauqeer
de Souza, Dziedzom K.
Vinayak, Sumiti
Griffing, Sean M.
Poe, Amanda C.
Duah, Nancy O.
Ghansah, Anita
Asamoa, Kwame
Slutsker, Laurence
Wilson, Michael D.
Barnwell, John W.
Udhayakumar, Venkatachalam
Koram, Kwadwo A.
Publication Title: 
Malaria Journal

BACKGROUND: Chloroquine resistance (CR) decreased after the removal of chloroquine from national treatment guidelines in Malawi, Kenia and Tanzania. In this investigation the prevalence of the chloroquine resistance (CQR) conferring mutant pfcrt allele and its associated chromosomal haplotype were determined before and after the change in Gabonese national treatment guidelines from chloroquine (CQ) to artesunate plus amodiaquine (AQ) in 2003.

Author(s): 
Frank, Matthias
Lehners, Nicola
Mayengue, Pembe I.
Gabor, Julian
Dal-Bianco, Matthias
Kombila, David U.
Ngoma, Ghyslain Mombo
Supan, Christian
Lell, Bertrand
Ntoumi, Francine
Grobusch, Martin P.
Dietz, Klaus
Kremsner, Peter G.
Publication Title: 
Malaria Journal

BACKGROUND: Sulphadoxine-pyrimethamine (SP) resistance is now widespread throughout east and southern Africa and artemisinin compounds in combination with synthetic drugs (ACT) are recommended as replacement treatments by the World Health Organization (WHO). As well as high cure rates, ACT has been shown to slow the development of resistance to the partner drug in areas of low to moderate transmission. This study looked for evidence of protection of the partner drug in a high transmission African context.

Author(s): 
Malisa, Allen L.
Pearce, Richard J.
Mutayoba, Ben M.
Abdullah, Salim
Mshinda, Hassan
Kachur, Patrick S.
Bloland, Peter
Roper, Cally
Publication Title: 
Science (New York, N.Y.)

Evolving resistance to artemisinin-based compounds threatens to derail attempts to control malaria. Resistance has been confirmed in western Cambodia and has recently emerged in western Thailand, but is absent from neighboring Laos. Artemisinin resistance results in reduced parasite clearance rates (CRs) after treatment. We used a two-phase strategy to identify genome region(s) underlying this ongoing selective event.

Author(s): 
Cheeseman, Ian H.
Miller, Becky A.
Nair, Shalini
Nkhoma, Standwell
Tan, Asako
Tan, John C.
Al Saai, Salma
Phyo, Aung Pyae
Moo, Carit Ler
Lwin, Khin Maung
McGready, Rose
Ashley, Elizabeth
Imwong, Mallika
Stepniewska, Kasia
Yi, Poravuth
Dondorp, Arjen M.
Mayxay, Mayfong
Newton, Paul N.
White, Nicholas J.
Nosten, François
Ferdig, Michael T.
Anderson, Timothy J. C.

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