Molecular Weight

Publication Title: 
Journal of Separation Science

This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions.

Author(s): 
Zou, Deng-lang
Chen, Tao
Li, Hong-Mei
Chen, Chen
Zhao, Jing-yang
Li, Yu-lin
Publication Title: 
Journal of Agricultural and Food Chemistry

Dry bean consumption has been reported to be associated with reduced risk for a number of chronic diseases including cancer. The extent to which these benefits are associated with primary versus secondary plant metabolites is not known. The work reported herein focuses on low molecular weight secondary metabolites and uses longevity extension of wild-type Caenorhabditis elegans nematodes as a surrogate marker for human health benefits.

Author(s): 
Mensack, Meghan M.
Fitzgerald, Vanessa K.
Lewis, Matthew R.
Thompson, Henry J.
Publication Title: 
Cell

We have analyzed the de novo telomere synthesis catalyzed by the enzyme telomere terminal transferase (telomerase) from Tetrahymena. Oligonucleotides representing the G-rich strand of telomeric sequences from five different organisms specifically primed the addition of TTGGGG repeats in vitro, suggesting that primer recognition may involve a DNA structure unique to these oligonucleotides. The sequence at the 3' end of the oligonucleotide primer specified the first nucleotide added in the reaction.

Author(s): 
Greider, C. W.
Blackburn, E. H.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Blackburn, E. H.
Publication Title: 
Cell

Macronuclear DNA from the protozoan G. chattoni, a holotrichous ciliate, was analyzed. Most, if not all, of the macronuclear DNA is subchromosomal, ranging in size from above 100 kb down to 2.1 kb, with molecules in the lower molecular weight range being resolvable by gel electrophoresis into reproducible, specific, discrete size classes. A prominent class of linear 9.3 kb molecules consists of single free rRNA genes. Upon denaturation of total macronuclear DNA was found as single-stranded circles.

Author(s): 
Katzen, A. L.
Cann, G. M.
Blackburn, E. H.
Publication Title: 
The Journal of Biological Chemistry
Author(s): 
Davidson, B. E.
Blackburn, E. H.
Dopheide, T. A.
Publication Title: 
Journal of Molecular Biology
Author(s): 
Blackburn, E. H.
Publication Title: 
Nature

Telomeres, the ends of eukaryotic chromosomes, have long been recognized as specialized structures. Their stability compared with broken ends of chromosomes suggested that they have properties which protect them from fusion, degradation or recombination. Furthermore, a linear DNA molecule such as that of a eukaryotic chromosome must have a structure at its ends which allows its complete replication, as no known DNA polymerase can initiate synthesis without a primer.

Author(s): 
Shampay, J.
Szostak, J. W.
Blackburn, E. H.
Publication Title: 
Cell

Macronuclear DNA from the protozoan G. chattoni, a holotrichous ciliate, was analyzed. Most, if not all, of the macronuclear DNA is subchromosomal, ranging in size from above 100 kb down to 2.1 kb, with molecules in the lower molecular weight range being resolvable by gel electrophoresis into reproducible, specific, discrete size classes. A prominent class of linear 9.3 kb molecules consists of single free rRNA genes. Upon denaturation of total macronuclear DNA was found as single-stranded circles.

Author(s): 
Katzen, A. L.
Cann, G. M.
Blackburn, E. H.
Publication Title: 
Antimicrobial Agents and Chemotherapy

Malaria is one of the most significant causes of infectious disease in the world. The search for new antimalarial chemotherapies has become increasingly urgent due to the parasites' resistance to current drugs. Ellagic acid is a polyphenol found in various plant products. In this study, antimalarial properties of ellagic acid were explored. The results obtained have shown high activity in vitro against all Plasmodium falciparum strains whatever their levels of chloroquine and mefloquine resistance (50% inhibitory concentrations ranging from 105 to 330 nM).

Author(s): 
Soh, Patrice Njomnang
Witkowski, Benoit
Olagnier, David
Nicolau, Marie-Laure
Garcia-Alvarez, Maria-Concepcion
Berry, Antoine
Benoit-Vical, Françoise

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