NADPH-Ferrihemoprotein Reductase

Publication Title: 
Experimental Gerontology

Centenarians are people who escaped from major common diseases, including cancer, and reached the extreme limits of human life-span. The analysis of demographic data indicates that cancer incidence and mortality show a levelling off around the age of 85-90 years, and suggests that oldest old people and centenarians are protected from cancer onset and progression.

Author(s): 
BonafË, Massimiliano
Barbi, Cristiana
Storci, Gianluca
Salvioli, Stefano
Capri, Miriam
Olivieri, Fabiola
Valensin, Silvana
Monti, Daniela
Gonos, Efstathios S.
De Benedictis, Giovanna
Franceschi, Claudio
Publication Title: 
Journal of Environmental Pathology, Toxicology and Oncology: Official Organ of the International Society for Environmental Toxicology and Cancer

The effect of chlordimeform treatment on the hepatic microsomal drug metabolizing enzymes was examined in male and female rats following either acute or repeated treatment.

Author(s): 
Budris, D. M.
Yim, G. K.
Schnell, R. C.
Publication Title: 
Genetics and molecular research: GMR

Finding an efficient and affordable treatment against malaria is still a challenge for medicine. Artemisinin is an effective anti-malarial drug isolated from Artemisia annua. However, the artemisinin content of A. annua is very low. We used transgenic technology to increase the artemisinin content of A. annua by overexpressing cytochrome P450 monooxygenase (cyp71av1) and cytochrome P450 reductase (cpr) genes. CYP71AV1 is a key enzyme in the artemisinin biosynthesis pathway, while CPR is a redox partner for CYP71AV1. Eight independent transgenic A.

Author(s): 
Shen, Q.
Chen, Y. F.
Wang, T.
Wu, S. Y.
Lu, X.
Zhang, L.
Zhang, F. Y.
Jiang, W. M.
Wang, G. F.
Tang, K. X.
Publication Title: 
Cancer Research

In order to elucidate the enzymic basis of nitrosamine metabolism, the in vitro metabolism of nitrosamines by rat liver microsomes and the effects of fasting on the microsomal enzymes have been studied. Fasting for 1 to 3 days causes a 2- to 3-fold enhancement of the reduced nicotinamide adenine dinucleotide phosphate-dependent nitrosodimethylamine demethylase (NDMAD) activity. The cytochrome P-450 content and the activities of reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 reductase and benzphetamine demethylase, however, are only modestly increased.

Author(s): 
Tu, Y. Y.
Yang, C. S.
Publication Title: 
Chemico-Biological Interactions

The effects of acetone and isopropanol on the microsomal monooxygenase system have been investigated to study the role of this enzyme system in the metabolism of nitrosamines. Treatment of rats with acetone or isopropanol (2.5-5 ml/kg, i.g.) causes a 3-4.5-fold enhancement in the NADPH-dependent nitrosodimethylamine demethylase (NDMAd) activity. This is accompanied by only moderate increases in the gross cytochrome P-450 (P-450) content and NADPH-cytochrome c reductase activity.

Author(s): 
Tu, Y. Y.
Peng, R.
Chang, Z. F.
Yang, C. S.
Publication Title: 
Journal of the National Cancer Institute

The effects of riboflavin deficiency on the metabolism of N-nitrosodimethylamine [(DMN) CAS: 62-75-9] and other nitrosamines were examined in rats. After weanling rats were put on a riboflavin-deficient diet, the development of the deficiency was monitored by the growth rate and the erythrocyte glutathione reductase activation coefficient. In the riboflavin-deficient rats, the liver microsomal NADPH-cytochrome c reductase activity was lower but the cytochrome P450 content was higher than that of the control. The metabolism of DMN was dependent on the severity of the deficiency.

Author(s): 
Wang, T.
Miller, K. W.
Tu, Y. Y.
Yang, C. S.
Publication Title: 
Phytotherapy research: PTR

The influence of Liv.100 on the hepatotoxicity of antituberculosis drugs [isoniazid (INH), rifampicin (RMP) pyrazinamide (PZA)] was studied in male albino rats. INH, RMP, and PZA were proved to be the most hepatotoxic. Rats were treated with antituberculosis drugs daily for a period of 6 weeks by intragastric administration. The combined use of antituberculosis drugs elevated the levels of cytochrome P-450 and cytochrome-b5. A significant increase was observed in the levels of NADPH-cytochrome P-450 reductase and NADH-cytochrome-b5 reductases after antitubercular drug administration.

Author(s): 
Saraswathy, S. D.
Shyamala Devi, C. S.
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

Cytochrome P450 reductase (POR) is a microsomal electron transport protein essential to cytochrome P450-mediated drug metabolism and sterol and bile acid synthesis. The conditional deletion of hepatic POR gene expression in mice results in a marked decrease in plasma cholesterol levels counterbalanced by the accumulation of triglycerides in lipid droplets in hepatocytes.

Author(s): 
Porter, Todd D.
Banerjee, Subhashis
Stolarczyk, Elzbieta I.
Zou, Ling
Publication Title: 
The Journal of Steroid Biochemistry and Molecular Biology

7-Dehydrocholesterol reductase (DHCR7) catalyzes the final step in cholesterol synthesis. The enzyme utilizes NADPH as a source of electrons and has been reported to require NADPH-cytochrome P450 reductase (POR) as its redox partner. To test this hypothesis, microsomes were prepared from the livers of mice in which hepatic cytochrome P450 reductase expression was extinguished during maturation. These microsomes contained negligible levels of POR but had 2.5-fold greater DHCR7 activity than did microsomes from wild-type mice.

Author(s): 
Zou, Ling
Li, Li
Porter, Todd D.
Publication Title: 
Drug Metabolism and Disposition: The Biological Fate of Chemicals

This is a report on a symposium sponsored by the American Society for Pharmacology and Experimental Therapeutics and held at the Experimental Biology 2012 meeting in San Diego, California, on April 25, 2012. The symposium speakers summarized and critically evaluated our current understanding of the physiologic, pharmacological, and toxicological roles of NADPH-cytochrome P450 oxidoreductase (POR), a flavoprotein involved in electron transfer to microsomal cytochromes P450 (P450), cytochrome b(5), squalene mono-oxygenase, and heme oxygenase.

Author(s): 
Riddick, David S.
Ding, Xinxin
Wolf, C. Roland
Porter, Todd D.
Pandey, Amit V.
Zhang, Qing-Yu
Gu, Jun
Finn, Robert D.
Ronseaux, Sebastien
McLaughlin, Lesley A.
Henderson, Colin J.
Zou, Ling
Flück, Christa E.

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