Osteosarcoma

Publication Title: 
Journal of Ethnopharmacology

A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([(3)H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining).

Author(s): 
Saleem, Ammar
Husheem, Michael
Härkönen, Pirkko
Pihlaja, Kalevi
Publication Title: 
Clinical Orthopaedics and Related Research

BACKGROUND: Orthopaedic oncologists often must address leg-length discrepancy after resection of tumors in growing patients with osteosarcoma. There are various alternatives to address this problem. We describe a three-stage procedure: (1) temporary arthrodesis, (2) lengthening by Ilizarov apparatus, and (3) tumor prosthesis.

Author(s): 
Kong, Chang-Bae
Lee, Soo-Yong
Jeon, Dae-Geun
Publication Title: 
Journal of Zhejiang University. Science. B

This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo.

Author(s): 
Xu, Qiang
Li, Zhao-Xu
Peng, Hui-Qin
Sun, Zheng-Wang
Cheng, Rui-Lin
Ye, Zhao-Ming
Li, Wei-Xu
Publication Title: 
International Journal of Oncology

We previously established a bioassay method to screen for compounds that activate the promoter activity of p21(WAF1/CIP1), a potent inhibitor of cyclin-dependent kinases, in a p53-independent manner. As an activator of p21(WAF1/CIP1) promoter activity, we isolated cryptolepine (CLP: 5-methyl indolo (2,3b)-quiniine), an indoloquinoline alkaloid, from the traditional Ayurvedic medicinal plant Sida cordifolia. We show here that CLP induces the expression of p21(WAF1/CIP1) with growth arrest in p53-mutated human osteosarcoma MG63 cells.

Author(s): 
Matsui, Taka-aki
Sowa, Yoshihiro
Murata, Hiroaki
Takagi, Koichi
Nakanishi, Ryoko
Aoki, Shunji
Yoshikawa, Masayuki
Kobayashi, Motomasa
Sakabe, Tomoya
Kubo, Toshikazu
Sakai, Toshiyuki
Publication Title: 
Clinical Cancer Research: An Official Journal of the American Association for Cancer Research

PURPOSE: Osteosarcoma is the most common primary malignancy of bone. The long-term survival of osteosarcoma patients hinges on our ability to prevent and/or treat recurrent and metastatic lesions. Here, we investigated the activation of peroxisome proliferator-activated receptor gamma (PPARgamma) and retinoid receptors as a means of differentiation therapy for human osteosarcoma. EXPERIMENTAL DESIGN: We examined the endogenous expression of PPARgamma and retinoid receptors in a panel of osteosarcoma cells.

Author(s): 
He, Bai-Cheng
Chen, Liang
Zuo, Guo-Wei
Zhang, Wenli
Bi, Yang
Huang, Jiayi
Wang, Yi
Jiang, Wei
Luo, Qing
Shi, Qiong
Zhang, Bing-Qiang
Liu, Bo
Lei, Xia
Luo, Jinyong
Luo, Xiaoji
Wagner, Eric R.
Kim, Stephanie H.
He, Connie J.
Hu, Yawen
Shen, Jikun
Zhou, Qixin
Rastegar, Farbod
Deng, Zhong-Liang
Luu, Hue H.
He, Tong-Chuan
Haydon, Rex C.
Publication Title: 
Cancer Biology & Therapy

Arsenic trioxide (As(2)O(3)) is an active ingredient in traditional Chinese medicine. Recent studies showed that it causes apoptosis in several cancer cells. However, research of As(2)O(3) in osteosarcoma is sparse. In our present study, an inhibitory effect of As(2)O(3) on osteosarcoma cell adhesion and metastasis was observed with a cell adhesion, migration and invasion test. The impact of As(2)O(3) on the activities of MMP-9 and MAPK pathway-related downstream factors was analyzed by western blotting.

Author(s): 
Tingting, Ren
Wei, Guo
Changliang, Peng
Xinchang, Lu
Yi, Yang
Publication Title: 
African journal of traditional, complementary, and alternative medicines: AJTCAM / African Networks on Ethnomedicines

This paper mainly examined the anti-osteosarcoma activity of total flavonoids extract from traditional Chinese medicine, nidus vespae. Orthogonal design was used to design the extraction process of total flavonoids. L(3(4)) orthogonal test was performed and the extracts obtained by three optimal extraction processes were used for anti-tumour activity screening in order to determine the optimal anti-tumour effective component of nidus vespae. MTT assay was used to investigate the effect of nidus vespae extract on proliferation activity of osteosarcoma cells.

Author(s): 
Jiang, Zhenhuan
Jiang, Jiannong
Wang, Qiang
Zhang, Leiyan
Wu, Chenguang
Yang, Huilin
Publication Title: 
African journal of traditional, complementary, and alternative medicines: AJTCAM / African Networks on Ethnomedicines

This paper mainly studied the extraction process of traditional Chinese medicine Aristolochia mollissima Hance (Aristolochiaceae) and the inhibitory effect of its extracts on osteosarcoma HOS cells. The extraction process included the ultrasonic extraction method, heat reflux method and decoction method to obtain three different extracts. MTT assay was used to test the effect of the extracts on proliferation of HOS cells, to compare the degree of inhibitory activity of three extracts, and to calculate cell survival rates.

Author(s): 
Yu, Yu
Bo, Zhao
Chao, He
Minghua, Zhang
Publication Title: 
Explore (New York, N.Y.)

Our purpose is to discuss the study design and innovative approaches that led to finding significant effects of one energy medicine therapy, Therapeutic Touch (TT), on cells. In the original published studies, TT was shown to significantly increase human osteoblast DNA synthesis, differentiation, and mineralization; increase in a dose-dependent manner the growth of other human cell types; and decrease the differentiation and mineralization of a human osteosarcoma-derived cell line.

Author(s): 
Monzillo, Eloise
Gronowicz, Gloria
Publication Title: 
Explore (New York, N.Y.)

Our purpose is to discuss the study design and innovative approaches that led to finding significant effects of one energy medicine therapy, Therapeutic Touch (TT), on cells. In the original published studies, TT was shown to significantly increase human osteoblast DNA synthesis, differentiation, and mineralization; increase in a dose-dependent manner the growth of other human cell types; and decrease the differentiation and mineralization of a human osteosarcoma-derived cell line.

Author(s): 
Monzillo, Eloise
Gronowicz, Gloria

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