Random Amplified Polymorphic DNA Technique

Publication Title: 
Molecular Biology Reports

Terminalia trees are being over-exploited because of their medicinal and economical importance leading to loss of valuable genetic resources. For sustainable utilization and conservation, assessment of genetic diversity therefore becomes imperative. We report a comprehensive first study on estimation and analysis of genetic variation through Amplified fragment length polymorphism (AFLP), inter simple sequence repeat polymorphism (ISSR) and random amplification of polymorphic DNA (RAPD) across three species of Terminalia.

Author(s): 
Sarwat, Maryam
Das, Sandip
Srivastava, Prem S.
Publication Title: 
Biological & Pharmaceutical Bulletin

Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon.

Author(s): 
Dnyaneshwar, Warude
Preeti, Chavan
Kalpana, Joshi
Bhushan, Patwardhan
Publication Title: 
Biological & Pharmaceutical Bulletin

Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon.

Author(s): 
Dnyaneshwar, Warude
Preeti, Chavan
Kalpana, Joshi
Bhushan, Patwardhan
Publication Title: 
Biotechnology and Applied Biochemistry

Zingiber officinale Roscoe (common or culinary ginger) is an official drug in Ayurvedic, Indian herbal, Chinese, Japanese, African and British Pharmacopoeias. The objective of the present study was to develop DNA-based markers that can be applied for the identification and differentiation of the commercially important plant Z. officinale Roscoe from the closely related species Zingiber zerumbet (pinecone, bitter or 'shampoo' ginger) and Zingiber cassumunar [cassumunar or plai (Thai) ginger].

Author(s): 
Chavan, Preeti
Warude, Dnyaneshwar
Joshi, Kalpana
Patwardhan, Bhushan
Publication Title: 
Fitoterapia

Identification of medicinal plants by their molecular signature is a fast growing tool. The identification of Desmodium gangeticum (L.) DC. (Shalparni, a constituent of Ayurvedic formulation "Dashmoolarishtha") was carried out using genomic approach. Authentic samples of D. gangeticum(L.) DC., D. velutinum (Willd.) DC. and D. triflorum (L.) DC. were analyzed and compared to commercial samples of various origin. Within twenty primers used, eleven gave 223 RAPD fragments.

Author(s): 
Irshad, Saba
Singh, Jyotsna
Kakkar, Poonam
Mehrotra, Shanta
Publication Title: 
Fitoterapia

Withania somnifera is one of the most important medicinal plants of Ayurveda and finds extensive uses in Indian traditional herbal preparations. In this investigation, selected accessions of the plant were examined for diversity through RAPDs, isoenzymes, polypeptide polymorphism and withanolide profiles. The accessions clustered together with respect to their characteristic profile of major withanolides and represented withaferin A, withanone, withanolide D or withanolide A rich groups.

Author(s): 
Chaurasiya, Narayan D.
Sangwan, Rajender S.
Misra, Laxmi N.
Tuli, Rakesh
Sangwan, Neelam S.
Publication Title: 
Brazilian Journal of Biology = Revista Brasleira De Biologia

Maytenus ilicifolia is a medicinal plant largely used in the South Brazilian folk medicine. The aim of this study was to quantify the intra and inter populational genetic variability in three populations of M. ilicifolia, focusing on the genetic conservation of this species, which has been threatened by anthropic action. RAPD (Random Amplified Polymorphic DNA) markers were used to analyze 30 plants of each of the three populations collected in the Alto Uruguai Gaúcho region. Fourteen selected primers generated a total of 158 bands, 71.5% of which were polymorphic.

Author(s): 
Mossi, A. J.
Cansian, R. L.
Leontiev-Orlov, O.
Zanin, E. M.
Oliveira, C. H.
Cechet, M. L.
Carvalho, A. Z.
Echeverrigaray, S.
Publication Title: 
Acta Pharmacologica Sinica

AIM: An efficient, precise, and sensitive method for identifying Atractylodes plants has been established and will contribute significantly to quality control and scientific analysis in Chinese traditional medicine. METHODS: Twenty primers were applied for setting up the RAPD (randomly amplified polymorphic DNA) markers of Atractylodes plants, Atractylodes lancea DC (A lancea DC), Atractylodes japonica Koidz (A japonica K), and Atractylodes ovata DC (A ovata DC). The primer OPF03, OPF05, and OPF14 could discriminate them successfully.

Author(s): 
Chen, K. T.
Su, Y. C.
Lin, J. G.
Hsin, L. H.
Su, Y. P.
Su, C. H.
Li, S. Y.
Cheng, J. H.
Mao, S. J.
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