ras Proteins

Publication Title: 
Experimental Cell Research

Soft tissue sarcomas (STS) are characterized by co-participation of several epigenetic and genetic events during tumorigenesis. Having bypassed cellular senescence barriers during oncogenic transformation, the factors further affecting growth rate of STS cells remain poorly understood.

Author(s): 
Becerikli, Mustafa
Jacobsen, Frank
Rittig, Andrea
Kˆhne, Wiebke
Nambiar, Sandeep
Mirmohammadsadegh, Alireza
Stricker, Ingo
Tannapfel, Andrea
Wieczorek, Stefan
Epplen, Joerg Thomas
Tilkorn, Daniel
Steinstraesser, Lars
Publication Title: 
British Journal of Pharmacology

BACKGROUND AND PURPOSE: Artemisinin and its derivatives exhibit potent immunosuppressive activity. The purpose of the current study was to examine the immunosuppressive activity of artemether directly on T lymphocytes and to explore its potential mode of action. EXPERIMENTAL APPROACH: In vitro, T-cell proliferation was measured using [(3)H]-thymidine incorporation assay in cells stimulated with ConA, alloantigen and anti-CD3 antibody. CFSE-labeled cell division and cell cycle distribution were monitored by flow cytometry.

Author(s): 
Wang, J.-X.
Tang, W.
Shi, L.-P.
Wan, J.
Zhou, R.
Ni, J.
Fu, Y.-F.
Yang, Y.-F.
Li, Y.
Zuo, J.-P.
Publication Title: 
Hepatology (Baltimore, Md.)

Glycine N-methyltransferase (GNMT) is the main enzyme responsible for catabolism of excess hepatic S-adenosylmethionine (SAMe). GNMT is absent in hepatocellular carcinoma (HCC), messenger RNA (mRNA) levels are significantly lower in livers of patients at risk of developing HCC, and GNMT has been proposed to be a tumor-susceptibility gene for liver cancer. The identification of several children with liver disease as having mutations of the GNMT gene further suggests that this enzyme plays an important role in liver function.

Author(s): 
Martínez-Chantar, M. Luz
Vázquez-Chantada, Mercedes
Ariz, Usue
Martínez, Núria
Varela, Marta
Luka, Zigmund
Capdevila, Antonieta
Rodríguez, Juan
Aransay, Ana M.
Matthiesen, Rune
Yang, Heping
Calvisi, Diego F.
Esteller, Manel
Fraga, Mario
Lu, Shelly C.
Wagner, Conrad
Mato, José M.
Publication Title: 
Diabetes

OBJECTIVE: Experimental infection of rats with human adenovirus type 36 (Ad-36) promotes adipogenesis and improves insulin sensitivity in a manner reminiscent of the pharmacologic effect of thiozolinediones. To exploit the potential of the viral proteins as a therapeutic target for treating insulin resistance, this study investigated the ability of Ad-36 to induce metabolically favorable changes in human adipose tissue. RESEARCH DESIGN AND METHODS: We determined whether Ad-36 increases glucose uptake in human adipose tissue explants.

Author(s): 
Rogers, Pamela M.
Mashtalir, Nazar
Rathod, Miloni A.
Dubuisson, Olga
Wang, Zhong
Dasuri, Kumar
Babin, Scott
Gupta, Alok
Markward, Nathan
Cefalu, William T.
Dhurandhar, Nikhil V.
Publication Title: 
Carcinogenesis

Previous studies have shown that stearate (C18:0), a dietary long-chain saturated fatty acid, inhibits breast cancer cell neoplastic progression; however, little is known about the mechanism modulating these processes. We demonstrate that stearate, at physiological concentrations, inhibits cell cycle progression in human breast cancer cells at both the G(1) and G(2) phases. Stearate also increases cell cycle inhibitor p21(CIP1/WAF1) and p27(KIP1) levels and concomitantly decreases cyclin-dependent kinase 2 (Cdk2) phosphorylation.

Author(s): 
Li, Chuanyu
Zhao, Xiangmin
Toline, Eric C.
Siegal, Gene P.
Evans, Lynda M.
Ibrahim-Hashim, Arig
Desmond, Renee A.
Hardy, Robert W.
Publication Title: 
Gastroenterology

BACKGROUND & AIMS: Patients with cirrhosis are at high risk for developing hepatocellular carcinoma (HCC), and their liver tissues have abnormal levels of S-adenosylmethionine (SAMe). Glycine N-methyltransferase (GNMT) catabolizes SAMe, but its expression is down-regulated in HCC cells. Mice that lack GNMT develop fibrosis and hepatomas and have alterations in signaling pathways involved in carcinogenesis. We investigated the role of GNMT in human HCC cell lines and in liver carcinogenesis in mice.

Author(s): 
Martínez-López, Nuria
García-Rodríguez, Juan L.
Varela-Rey, Marta
Gutiérrez, Virginia
Fernández-Ramos, David
Beraza, Naiara
Aransay, Ana M.
Schlangen, Karin
Lozano, Juan Jose
Aspichueta, Patricia
Luka, Zigmund
Wagner, Conrad
Evert, Matthias
Calvisi, Diego F.
Lu, Shelly C.
Mato, José M.
Martínez-Chantar, María L.
Publication Title: 
Cancer Prevention Research (Philadelphia, Pa.)

There is epidemiologic evidence that obesity increases the risk of cancers. Several underlying mechanisms, including inflammation and insulin resistance, are proposed. However, the driving mechanisms in pancreatic cancer are poorly understood. The goal of the present study was to develop a model of diet-induced obesity and pancreatic cancer development in a state-of-the-art mouse model, which resembles important clinical features of human obesity, for example, weight gain and metabolic disturbances.

Author(s): 
Dawson, David W.
Hertzer, Kathleen
Moro, Aune
Donald, Graham
Chang, Hui-Hua
Go, Vay Liang
Pandol, Steven J.
Lugea, Aurelia
Gukovskaya, Anna S.
Li, Gang
Hines, Oscar J.
Rozengurt, Enrique
Eibl, Guido
Publication Title: 
Genetics

Replicative capacity, which is the number of times an individual cell divides, is the measure of longevity in the yeast Saccharomyces cerevisiae. In this study, a process that involves signaling from the mitochondrion to the nucleus, called retrograde regulation, is shown to determine yeast longevity, and its induction resulted in postponed senescence. Activation of retrograde regulation, by genetic and environmental means, correlated with increased replicative capacity in four different S. cerevisiae strains.

Author(s): 
Kirchman, P. A.
Kim, S.
Lai, C. Y.
Jazwinski, S. M.
Publication Title: 
The Journal of Biological Chemistry

Weight control by exercise and dietary calorie restriction (DCR) has been associated with reduced cancer risk, but the underlying mechanisms are not well understood. This study was designed to compare the effects of weight loss by increasing physical activity or decreasing calorie intake on tumor promoter-induced Ras-MAPK and PI3K-Akt pathways. SENCAR mice were randomly assigned to one of the following five groups: ad libitum-fed sedentary control, ad libitum-fed exercise (AL+Exe), exercise but pair-fed at the amount as controls (PF+Exe), 20% DCR, and 20% DCR plus exercise (DCR+Exe).

Author(s): 
Xie, Linglin
Jiang, Yu
Ouyang, Ping
Chen, Jie
Doan, Hieu
Herndon, Betty
Sylvester, Jessica E.
Zhang, Ke
Molteni, Agostino
Reichle, Marie
Zhang, Rongqing
Haub, Mark D.
Baybutt, Richard C.
Wang, Weiqun
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