rho GTP-Binding Proteins

Publication Title: 
FEBS letters

Sir2 mediates lifespan extension in lower eukaryotes but whether its mammalian homolog, sirtuin 1, silent mating type information regulation 2 homolog (SIRT1), is a longevity protein is controversial. We stably introduced the SIRT1 gene into human vascular smooth muscle cells (SMCs) and observed minimal extension of replicative lifespan. However, SIRT1 activity was found to be exquisitely dependent on nicotinamide phosphoribosyltransferase (Nampt) activity.

Author(s): 
Ho, Cynthia
van der Veer, Eric
Akawi, Oula
Pickering, J. Geoffrey
Publication Title: 
PloS One

Withaferin A (WA), a promising anticancer constituent of Ayurvedic medicinal plant Withania somnifera, inhibits growth of MDA-MB-231 and MCF-7 human breast cancer cells in culture and MDA-MB-231 xenografts in vivo in association with apoptosis induction, but the mechanism of cell death is not fully understood. We now demonstrate, for the first time, that WA-induced apoptosis is mediated by reactive oxygen species (ROS) production due to inhibition of mitochondrial respiration.

Author(s): 
Hahm, Eun-Ryeong
Moura, Michelle B.
Kelley, Eric E.
Van Houten, Bennett
Shiva, Sruti
Singh, Shivendra V.
Publication Title: 
PloS One

Withaferin A (WA), a promising anticancer constituent of Ayurvedic medicinal plant Withania somnifera, inhibits growth of MDA-MB-231 and MCF-7 human breast cancer cells in culture and MDA-MB-231 xenografts in vivo in association with apoptosis induction, but the mechanism of cell death is not fully understood. We now demonstrate, for the first time, that WA-induced apoptosis is mediated by reactive oxygen species (ROS) production due to inhibition of mitochondrial respiration.

Author(s): 
Hahm, Eun-Ryeong
Moura, Michelle B.
Kelley, Eric E.
Van Houten, Bennett
Shiva, Sruti
Singh, Shivendra V.
Publication Title: 
Proceedings of the National Academy of Sciences of the United States of America

Huntington's disease (HD) is a neurodegenerative disease caused by polyglutamine (polyQ) expansion in the protein huntingtin (htt). Pathogenesis in HD seems to involve the formation of neuronal intranuclear inclusions and the abnormal regulation of transcription and signal transduction. To identify previously uncharacterized htt-interacting proteins in a simple model system, we used a yeast two-hybrid screen with a Caenorhabditis elegans activation domain library. We found a predicted SH3 domain protein (K08E3.3b) that interacts with N-terminal htt in two-hybrid tests.

Author(s): 
Holbert, Sébastien
Dedeoglu, Alpaslan
Humbert, Sandrine
Saudou, Frédéric
Ferrante, Robert J.
Neri, Christian
Publication Title: 
Clinical Cancer Research: An Official Journal of the American Association for Cancer Research

Alteration of actin polymerization and loss of actin filaments is a marker of cellular dedifferentiation and early malignant transformation. To study this phenomenon, an in vitro human urothelial model consisting of two cell lines, HUC-PC and MC-T11, were incorporated into the study design. These two cell lines have different malignant transformation potential. The effect of green tea extract (GTE), a potential anticancer agent, on actin remodeling was investigated.

Author(s): 
Lu, Qing-Yi
Jin, Yu-Sheng
Pantuck, Allan
Zhang, Zuo-Feng
Heber, David
Belldegrun, Arie
Brooks, Mai
Figlin, Robert
Rao, Jianyu
Publication Title: 
Carcinogenesis

Previous studies have shown that stearate (C18:0), a dietary long-chain saturated fatty acid, inhibits breast cancer cell neoplastic progression; however, little is known about the mechanism modulating these processes. We demonstrate that stearate, at physiological concentrations, inhibits cell cycle progression in human breast cancer cells at both the G(1) and G(2) phases. Stearate also increases cell cycle inhibitor p21(CIP1/WAF1) and p27(KIP1) levels and concomitantly decreases cyclin-dependent kinase 2 (Cdk2) phosphorylation.

Author(s): 
Li, Chuanyu
Zhao, Xiangmin
Toline, Eric C.
Siegal, Gene P.
Evans, Lynda M.
Ibrahim-Hashim, Arig
Desmond, Renee A.
Hardy, Robert W.
Publication Title: 
International Journal of Oncology

Soybean isoflavones have been used as a potential preventive agent in anticancer research for many years. Genistein is one of the most active flavonoids in soybeans. Accumulating evidence suggests that genistein alters a variety of biological processes in estrogen-related malignancies, such as breast and prostate cancers. However, the molecular mechanism of genistein in the prevention of human colon cancer remains unclear. Here we attempted to elucidate the anticarcinogenic mechanism of genistein in human colon cancer cells.

Author(s): 
Zhang, Zhiyu
Wang, Chong-Zhi
Du, Guang-Jian
Qi, Lian-Wen
Calway, Tyler
He, Tong-Chuan
Du, Wei
Yuan, Chun-Su
Publication Title: 
PloS One

Cell adhesion, migration and invasion are critical steps for carcinogenesis and cancer metastasis. Ganoderma lucidum, also called Lingzhi in China, is a traditional Chinese medicine, which exhibits anti-proliferation, anti-inflammation and anti-metastasis properties. Herein, GAEE, G. lucidum extract mainly contains ganoderiol A (GA), dihydrogenated GA and GA isomer, was shown to inhibit the abilities of adhesion and migration, while have a slight influence on that of invasion in highly metastatic breast cancer MDA-MB-231 cells at non-toxic doses.

Author(s): 
Wu, Guo-Sheng
Song, Yue-Lin
Yin, Zhi-Qi
Guo, Jia-Jie
Wang, Sheng-Peng
Zhao, Wen-Wen
Chen, Xiu-Ping
Zhang, Qing-Wen
Lu, Jin-Jian
Wang, Yi-Tao
Subscribe to RSS - rho GTP-Binding Proteins